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The German Federal Ministry of Research, Technologie and Space (BMFTR) and the Dutch organisation for knowledge and innovation in health, healthcare and well-being (ZonMw) launched a joint call for interdisciplinary research projects with a focus on method validation to further strengthen this field in Europe and to foster and consolidate a sustainable research cooperation between Germany and the Netherlands. This call aims to validate new approach methods in a regulatory context, with the ultimate goal to implement new animal-free, human relevant methods as OECD guidelines or establish qualified models to be used in efficacy testings for new pharmaceuticals.